Electron Diffraction Based Tilt Angle Measurements in Electron Tomography
نویسندگان
چکیده
منابع مشابه
Electron Diffraction Based Tilt Angle Measurements in Electron Tomography
Electron tomography can provide three dimensional (3D) visualization of nanoscale objects in a transmission electron microscope (TEM) from a tilt series of projected images. The accuracy of the 3D reconstruction depends on quality of input parameters, including accurate alignment in real space and accuracy of tilt and azimuth angle measurements. Here we present an electron diffraction-based in-...
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Fidelity of tomographic reconstructions is improved and reconstruction artifacts are reduced, without increasing the number of projections, by combining tilt series taken around two orthogonal axes. Test reconstructions were made from high-voltage EM of rat liver mitochondria in a 0.6 micron thick plastic section. A number of schemes for selecting tilt angles for the projections are compared. A...
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ژورنال
عنوان ژورنال: Microscopy and Microanalysis
سال: 2014
ISSN: 1431-9276,1435-8115
DOI: 10.1017/s1431927614005753